Taq PCR EasyMasterMix(5×1mL)
目录号:TPEM-5
产品概述
Taq PCR EasyMasterMix是我们为科研人员常规PCR实验提出的基础解决方案。
本产品包含Taq DNA聚合酶、dNTPs、MgCl2、反应缓冲液,浓度为1×。其中Taq DNA聚合酶是从带有Thermus aquaticus (Taq) DNA聚合酶基因的大肠杆菌中经诱导表达后分离提纯得到的,其分子量为94 KD。Taq DNA聚合酶具有5′→3′DNA聚合酶活性和5′→3′外切核酸酶活性,无3′→5′外切酶活性。在PCR反应中,Taq DNA聚合酶延伸速度为1-2 kb/min。
由于EasyMasterMix系列浓度均为1×,只需加入引物和模板即可进行扩增,无需补水。进一步减少了移液操作,最大限度提高了通量和结果的重现性。EasyMasterMix系列本身含有蓝紫色染料,扩增完成后可直接上样进行电泳检测。扩增产物3'端带A,可以直接用于T/A载体克隆。
使用说明
1.冰浴中彻底融化1×EasyMasterMix,混匀后短暂离心。
2.我们强烈建议客户购买本产品后分装成小份避免反复冻融。
3.以50μl反应体系为例,按照下表在0.2ml PCR管中加入下列成分
4. 短暂离心将反应液收集到管底。
5. 若基因扩增仪不防蒸发,请加1-2滴石蜡油。
6. 在PCR仪上执行以下程序(仅供参考)
注:在实际操作中需根据模板的结构、扩增片段的大小和引物的长短等具体情况,设定最佳的反应条件(温度、时间等)。
精选引用
对于使用我们的Taq DNA聚合酶的已发表研究感兴趣吗?
Pseudomonas spp. associated with tomato pith necrosis in the Salto area, Northwest Uruguay
European Journal of Plant Pathology | 19 Jan 2023 | DOI: https://doi.org/10.1007/s10658-023-02639-6
The PCR reactions contained 1X PCR buffer, 2.5 mM MgCl2, 0.4 mM of each dNTP, 0.4 μM of each primer, 1 U Taq polymerase (SBS Genetech Co., Ltd., China), and 1 μL of template DNA (100 ng μl−1). The PCR reaction was adjusted to a final volume of 25 μl with MQ water.
Sequencing of Norovirus in Southern, Nigeria: Prevalent Genotypes and Putative GII.4 Novel Recombinants among Children
Genetic Variation | 16 Dec 2020 | DOI: https://doi.org/10.5772/intechopen.94389
The RT-PCR used is a very sensitive method, it can detect as few as 5 x 106 copies per gram of stool sample. U-TaQ DNA polymerase (SBS genetech, Beijing, China), a high-fidelity thermostable enzyme that can withstand prolonged incubation at high temperature up to 95°C without significant loss of activity was used for this RT-PCR protocol.
Semi-nested polymerase chain reaction over blood culture in detection of bloodstream fungal infection in leukemic children with febrile neutropenia
Journal of Applied Hematology | 17 Nov 2020 | DOI: https://doi.org/10.4103/joah.joah_41_20
Taq polymerase enzymes and customized primers were procured from SBS Genetech Co., Ltd., China.
Expression, purification and initial characterization of human serum albumin domain I and its cysteine 34
PLOS ONE | 12 Oct 2020 | DOI: https://doi.org/10.1007/s10658-023-02639-6
Total RNA from HepG2 cells was isolated using the RNeasy mini kit (QIAGEN) following manufacturer’s instructions. Retrotranscription was performed using 2.5 μg of RNA, a specific HSA oligonucleotide (ATAAGCCTAAGGCAGCTTGACTGG) and Superscript II reverse transcriptase (Invitrogen). The full-coding sequence of HSA was PCR- amplified with U-Taq (SBS GeneTech)
Evaluation of Nested broad-range PCR for Pathogen Detection in Negative Blood Cultures
JOURNAL OF CLINICAL RESEARCH AND APPLIED MEDICINE | 6 Oct 2020 | DOI: https://doi.org/10.5530/jcram.2.2.11
Taq polymerase enzymes and customized primers were procured from SBS Genetech Co., Ltd., China.
Identificación por catálogo y detección molecular de bovinos Holstein portadores de braquiespina en Uruguay
Revista FAVE. Sección Ciencias veterinarias | 1 Aug 2020 | DOI: https://doi.org/10.14409/favecv.v19i2.9523
La reacción fue puesta a punto en un volumen final de 25µL conteniendo: 100ng de ADN genómico, 2.5µL de Buffer de PCR 10X (Mg2+: 20mM), 1µM de cada primer, 10mM dNTPs y 0.4µL U-Taq ADN polimerasa (SBS Genetech Co., Ltd., China).
仅用于研究,不用于治疗人类或动物
北京赛百盛基因技术有限公司是冷泉港实验室的长期赞助合作伙伴