The RT-PCR products were resolved on 2% (w/v) agarose gel and stained by GoldView (Beijing SBS Genetech Co Ltd.) (fig. S1A).
The RT-PCR products were resolved on 2% (wt/vol) agarose gel and stained by GoldView (Beijing SBS Genetech Co) (Figure S1C).
Peptide S160, S490, S497, the mutants of S160, S160-scrambled, Pep2-con, Pep2-S160, mutant of Pep2-S160 (SBS Genetech Co., Ltd).
The human CD147 mutants (K234R, K250R, K259R, and K261R) were generated from the eGFP-N1-CD147 plasmid using a site-directed mutagenesis kit (SBS).
Peptide A, B, C, D, PA (SBS Genetech Co., Ltd)
Nucleic Acids Research
HPLC purified oligonucleotides were procured from SBS Genetech, China.
The PPAR𝛾-S273A, S273D mutants were generated using Plenti-PPAR𝛾 plasmid as a template by polymerase chain reaction with a Muta-Direct Site-Directed Mutagenesis Kit (SBS Genetech Co., Ltd., China).
Annals of the Rheumatic Diseases
The formed digestion products were separated on 4% agarose gels and stained with GoldView (SBS Genetech Beijing, China).
Point mutations were made by Fast Mutagenesis Kit V2 (SBS Genetech).
Single-cell suspensions were pulsed with 1 μM hgp10025–23 (KVPRNQDWL) of immunograde quality, which was purchased from SBS (Beijing SBS Genetech Co. Ltd., Beijing, China).
Goldview DNA dye (Beijing SBS Genetech, cat. no. HGV-1)
Clinical Cancer Research
The restriction products were electrophoresed on a 3% agarose gel, and visualized by staining with Gold View (SBS Genetech).
The peptides used in this study (Mgpe9 and TD-1) were custom synthesized (>98% purity) by Beijing SBS Genetech.
The FITC-labeled control aptamer NK8 (bound to Mycobacterium tuberculosis) was synthesized by SBS Genetech Co., Ltd.
Peptides bradykinin and VEALYL (pentapeptide) were synthesized by SBS Genetech Co. Ltd. (Beijing, China).
Total RNA from S. gilvosporeus strains was prepared with Redzol reagent and extracted using the Total RNA extraction kit (SBS Genetech Co., Ltd.), according to the manufacturer's instructions.
Human G9a and p53 mutants were generated using Muta-direct Site-directed Mutagenesis Kit (Beijing SBS Genetech Co, Ltd, Beijing, China).
Single point mutants of TRPV1 were obtained by using Fast Mutagenesis Kit V2, (SBS Genetech Co.,Ltd., China) which were sequenced to confirm that site-directed mutagenesis was made.
Stem Cell Reports
Site-directed mutagenesis of selected putative seeding-sequence regions was performed using a Site-Directed Mutagenesis Kit (SBS Genetech).
Journal of the National Cancer Institute
Human pIgR mutants were generated using the Muta-direct Site-directed Mutagenesis kit (Beijing SBS Genetech Co, Ltd, Beijing, China).
The chimeric peptides and the SAH-EJ peptides as well as their fluorescently labelled
analogues were synthesized by Beijing SBS Genetech company (Beijing, China)