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      我们与世界各地的科学家们继续为生命科学领域增加了新的知识

      哈佛大学(美国)

      Harvard University

      The mutants were constructed by PCR-based site-directed mutagenesis (Muta-directTM kit, Beijing SBS Genetech Co., Ltd., China).

      伦敦帝国理工大学(英国)

      Imperial College London

      The wild CII263–272peptide (FKGE-QGPKGE), wild HA308–317peptide (YVKQNTLKLA), altered HA308–317peptide (YAKQATLALA), and irrelevant peptide (ALALTAQKAY) were synthesized by solid-phasetechniques (SBS Genetech). The purity was > 95%.

      新加坡国立大学(新加坡)

      National University of Singapore

      Ten peptides corresponding to the amino acids 20–39, 30–49, 42–61, 50–69, 60–79, 73–92, 88–108, 101–120, 111–130 and 124–144 of the Blo t 12.0101 protein were prepared by solid-phase synthesis with an automated peptide synthesizer, purified by high-performance liquid chromatography and (HPLC) and analysed by mass spectrometry(SBS Genetech, Beijing, China).

      康奈尔大学(美国)

      Cornell University

      The PCR products were checked for effective amplification through electrophoresis in 1% agarose gels containing 1:20 GoldView (SBS Genetech Co., Beijing, China).

      清华大学(中国)

      Tsinghua University

      The locked nucleic acids (LNA) targeting ZIKV sfRNA were chemically synthesized and HPLC purified by Beijing SBS Genetech Co., Ltd.

      密歇根大学(美国)

      University of Michigan

      These oligonucleotides were designed to amplify a 250-bp segment of GAPDH that serves as an internal standard. The primers were purchased from SBS Genetech Co. Ltd, Beijing, China.

      北京大学(中国)

      Peking University

      A Site‐Directed Mutagenesis Kit (SBS Genetech Co., Ltd) was then used to mutate the miR‐375 binding site in the 3′‐UTR of IGFBP3 and named as IGFBP3‐mutant‐type (MT) luciferase reporter plasmid.

      约翰霍普金斯大学(美国)

      Johns Hopkins University

      Fluorescein isothiocyanate (FITC)‐labeled peptides (N‐terminal) usedthroughout this manuscript were synthesized from SBS Genetech(Beijing, China), and purified using reversed phase analytical high‐performance liquid chromatography (>99% purity).

      香港大学(中国)

      The University of Hong Kong

      The PCR reaction mixture consisted of 2 µL DNA (about 15 ng), 2.5 µL of 10 × PCR buffer, 1.5 µL of 25 mmol/L MgCl2, 1.5 µL of 2.5 mmol/L dNTPs, 1.5 U of Taq DNA polymerase (SBS Genetech Co., China), 2.0 µL each of 2.5 µmol/L IT1F and IT2R primers (synthesized by SBS Genetech Co., China) in a final volume of 25 µL.

      加州大学伯克利分校(美国)

      University of California, Berkeley

      Amplified products were run on a 2% agarose gel with GoldView (SBS Genetech Co., Ltd., Beijing) for visualization.

      多伦多大学(加拿大)

      University of Toronto

      Point mutations of RokC114-185 were generated using the site-directed mutagenesis kit (SBS Genetech).

      京都大学(日本)

      Kyoto University

      Genomic DNA was extracted by Genome DNA Simax Kit (Beijing SBS Genetech Co., Ltd., China).

      麦吉尔大学(加拿大)

      McGill University

      Fluorescein isothiocyanate (FITC)‐labeled peptides (N‐terminal) used throughout this manuscript were synthesized from SBS Genetech(Beijing, China), and purified using reversed phase analytical high‐performance liquid chromatography (>99% purity).

      首尔国立大学(韩国)

      Seoul National University

      Each 25 µL reaction mixture contained 50 ng DNA, 5 pmol of each primer, 2 µL PCR buffer [100mM Tris (pH 8.3), 500 mM KCl, 15 mM MgCl2], 250 µM of each dNTPs and 0.5-1 unit Taq DNA polymerase (SBS Genetech Co, Beijing, China).

      浙江大学(中国)

      Zhejiang University

      Total RNA was then extracted using Redzol reagent (SBS Genetech Inc., Beijing, China)

      威斯康星大学麦迪逊分校(美国)

      University of Wisconsin, Madison

      Due to the high content of phenol and polysaccharides in pear leaves, genomic DNA was then purified using a purification kit from SBS Genetech Co. Ltd., China.

      上海交通大学(中国)

      Shanghai Jiao Tong University

      RNA was isolated using the Total RNA Isolation Kit (Beijing SBS Genetech Co., Ltd.) from mycelia of WT and its derivative ΔctcS mutant strains grown two days in fermentation medium.

      墨尔本大学(澳大利亚)

      University of Melbourne

      After DNA extraction, extracts were loaded on 1% agarose gel in 0.5x Tris-Acetate-EDTA (TAE) buffer with GoodView Nucleic Acid Stain (SBS Genetech Co., Ltd., China) and were electrophoresed (100 V).

      复旦大学(中国)

      Fudan University

      All primers were designed by Primer 5.0 software and synthesized by SBS Genetech (Beijing, China).

      新南威尔士大学(澳大利亚)

      The University of New South Wales

      We checked PCR ampli-cons with an electrophoresis in 0.7% agarose gelsstained using GoodView (SBS Genetech, Beijing, China)for visualization in UV light.

      英属哥伦比亚大学(加拿大)

      University of British Columbia

      Gap26 and10panx1 were synthetized by SBS Genetech (Beijing, China).

      中国科学技术大学(中国)

      University of Science and Technology of China

      The mutant luciferase reporter con-structs of HIF‐1 were constructed using Site‐DirectedMutagenesis Kit (SBS Genetech, Beijing, China).

      昆士兰大学(澳大利亚)

      University of Queensland

      Reverse-transcription of total RNA into the first-strand cDNA was performed by an RT-PreMix kit (SBS Genetech) and a universal dT3AP [oligo(dT)-containing adaptor primer], which were directly used as templates for RACE (3 rapid amplification of cDNA ends) with primers MeuICK-F1 and 3AP (Figure 1A).

      蒙纳士大学(澳大利亚)

      Monash University

      Desired mutations were introduced to the N-terminal double c-Myc–labeled human GLP-1R gene in pDONR201 (Invitrogen) via the Muta-directTM kit (Beijing SBS GenetechCo., Ltd., China)

      武汉大学(中国)

      Wuhan University

      Fluorescein isothiocyanate (FITC)‐labeled peptides (N‐terminal) used throughout this manuscript were synthesized from SBS Genetech(Beijing, China), and purified using reversed phase analytical high‐performance liquid chromatography (>99% purity).

      加州大学圣迭戈分校(美国)

      University of California, San Diego

      To make 5D, 5D-L, 5D-a, ASF-L and Tra2b-L series of constructs, sense and antisense oligos containing a full or truncated XhoI and EcoRI restriction sites were synthesized (SBS Genetech, Beijing) and diluted in the linker buffer (50 mM Tris–HCl pH 8.0, 100 mM NaCl, 1 mM EDTA) at a final concentration of 100 nM.

      苏黎世大学(瑞士)

      University of Zurich

      To make 5D, 5D-L, 5D-a, ASF-L and Tra2b-L series of constructs, sense and antisense oligos containing a full or truncated XhoI and EcoRI restriction sites were synthesized (SBS Genetech, Beijing) and diluted in the linker buffer (50 mM Tris–HCl pH 8.0, 100 mM NaCl, 1 mM EDTA) at a final concentration of 100 nM.

      马来亚大学(马来西亚)

      University of Malaya

      All the oligonucleotide probes and target were procured from SBS Genetech Co., Ltd (Beijing, PRC)

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