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Loop-mediated Isothermal Amplification (LAMP)
在赛百盛,我们基于新一代平台提供环介导等温扩增 (LAMP)的解决方案
Bst DNA/RNA Polymerase
我们的Bst DNA/RNA聚合酶是Bst聚合酶和极耐热逆转录酶(耐65°C)的混合物,适用于RNA的等温扩增反应。它可以检测低灵敏度RNA分子。在以RNA为模板的等温扩增实验中,推荐使用这种酶。此外,Bst DNA/RNA聚合酶还可以对DNA模板进行等温扩增。
更高的特异性
通过独特的阴性控制技术
更好的灵敏度
通过优化反应缓冲液
更低的假阳性率
通过对dUTP的强大识别能力
提供冻干版本
采用先进的冷冻干燥技术
LAMP解决方案
立即购买LAMP基础冻干微球¥720.00 - ¥3,600.00¥4,500.00立即购买RT-LAMP基础冻干微球¥720.00 - ¥3,600.00¥4,500.00立即购买LAMP基础冻干微球(防污染型)¥800.00 - ¥4,000.00¥5,000.00立即购买Bst DNA/RNA Polymerase¥1,380.00 - ¥9,300.00立即购买Bst DNA/RNA Polymerase (glycerol-free)¥3,480.00 - ¥9,300.00以创新驱动LAMP研究与应用加速
赛百盛助力LAMP研究人员在《Scientific Reports》等顶尖期刊发表高影响力论文,
推动科学进步与应用突破
European Food Research and Technology (Under Review) | 08 Aug 2025 | DOI: https://doi.org/10.21203/rs.3.rs-7079194/v1
Each reaction had a volume of 25 µL, comprising 1.6 µM each of FIP and BIP, 0.2 µM each of F3 and B3, 0.8 µM LF primer, 1.8 mM dNTPs (Thermo Scienti c, USA), 1x ThermoPol reaction buffer (20 mM Tris-HCl, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1% Triton X-100 (New England Biolabs, USA), 0.6 M betaine (TCI, USA), 8 U of Bst DNA polymerase (SBS Genetech Co, Beijing), and 1 µL of template DNA.
PLoS ONE | 11 May 2022 | DOI: https://doi.org/10.1371/journal.pone.0268340
They performed RT-LAMP with Bst DNA/RNA polymerase from SBS Genetech, and tested 67 negative and 96 positive crude NP samples [21]. They reported 100% sensitivity at Ct ≤ 25 and 53.1% sensitivity at Ct: 30–34 with 100% specificity. Compared with our results, their reported higher sensitivity at high Ct values is likely due to the polymerase used, which is extremely thermostable and also provides sensitive reverse transcriptase activity.
Scientific Reports | 08 Mar 2022 | DOI: https://doi.org/10.1038/s41598-022-06632-7
The mix contained in final concentration 8U Bst DNA/RNA Polymerase from SBS Genetech Co. (Beijing SBS Genetech Co., Ltd.), 10 × Bst DNA/RNA polymerase isothermal buffer (Mg2+ free), 7 mM Mg2+, 1.2 mM (each) dNTPs (Thermoscientific), 160 μM HNB dye and nuclease-free water up to 25 μl.
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