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Cat. No.: HLUDG-500 (for 500U)

Cat. No.: HLUDG-5k (for 5,000U)

For the standard version, please visit Uracil N-Glycosylase.

Description

Heat-Labile Uracil DNA Glycosylase can effectively hydrolyze uracil-glycosidic bonds in single- or double-stranded DNA, excising uracil and creating alkali-sensitive abasic sites in the DNA, which can be hydrolyzed by endonuclease, heat, or alkali treatment. The enzyme has no activity to RNA and is mainly used to prevent contamination in the PCR reaction system. The uracil DNA glycosylase from E.coli is relatively heat-resistant, and a small amount of uracil DNA glycosylase activity remains after being treated at 95°C for 10 min, which leads to the degradation of PCR products containing dU base. While the HL-UDG from psychrophilic marine bacteria is completely inactivated at 55°C for 5 min. Before PCR amplification, HL-UDG is added to the PCR reaction system, and the contamination in the system could be eliminated at 25°C for 10 min. HL-UDG will then be inactivated in the first step of denaturation (at 94°C) of the PCR cycle, preventing the degradation of the synthesized PCR products containing dU.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the release of 60 pmol uracil from the double-stranded DNA containing uracil at 37°C per minute.

Reaction Buffer

HL-UDG is compatible with most PCR reaction buffers, but its activity is inhibited at high ion concentrations (> 100 mm).

Storage Buffer

20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, , 50% Glycerol, 0.1% (w/v) Triton X-100, pH 7.5.

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing. 

Heat Inactivation

55°C，5-10min.

Only for research and not intended for treatment of humans or animals

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