Cas13 is a class II and type VI CRISPR system effector protein with two higher eukaryotes and prokaryotes nucleotide-binding (HEPN) binding domains. It is a novel CRISPR protease that can be used for targeted RNA cleavage. While Cas13 recognizes and cleaves target RNA under the guidance of guide RNA, its collateral cleavage activity is activated, which can efficiently cleave non-specific single-stranded RNA (ssRNA). The lyophilized version of Cas13a can be transported at room temperature, saving the high cost of dry ice transportation.
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Cat. No.: Cas13aLyo-100 (for 100pmol)
Cat. No.: Cas13aLyo-1k (for 1000pmol)
CRISPR/Cas13a can be used to detect RNA templates and amplify signals by designing RNA probes labeled with fluorescent groups or other markers at both ends. Common detection methods include real-time fluorescence and colloidal gold chromatography. At present, this system has been widely used in gene editing of microorganisms, plants and animals, and has great practical application value in molecular diagnosis.
This product is a recombinant protein. 1pmol=144 ng.
Lyophilized powder dissolution: Add enzyme-free H2O to dissolve, and it is recommended to dissolve it when it will be used. Add 50% enzyme-free glycerol to dissolve, and it can be stored at -20°C for 12 months. The recommended final concentration is 2pmol/μl.
Both Cas13a and 10×Buffer can be transported at room temperature.
The minimum shelf life is 1 year at 2-28°C for Cas13a and 1 year at -20°C for 10×Buffer.