Cas12a Nuclease (Lyophilized)
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Cat. No.: Cpf1Lyo-100 (for 100pmol)
Cat. No.: Cpf1Lyo-2k (for 2000pmol)
Description
Cas12a recognizes and cleaves target double-stranded DNA (dsDNA), and its collateral cleavage activity is activated, which can efficiently cleave non-specific single-stranded DNA (ssDNA). CRISPR/Cas12a can be used to detect DNA templates and amplify signals by designing DNA probes labeled with fluorescent groups or other markers at both ends. Common detection methods include real-time fluorescence and colloidal gold chromatography. GRNA specifically recognizes the target region, the CRISPR/Cas12a system is equivalent to a "secondary screening" of target DNA or DNA amplification products, so it has high specificity.
Components
Cpf1Lyo-100 (for 100pmol): Cas12a (20 pmol×5), 10×Buffer (500μl×1)
Cpf1Lyo-2k (for 2000pmol): Cas12a (2000 pmol×1), 10×Buffer (1ml×5)
Lyophilized powder dissolution: Add enzyme-free H2O to dissolve, and it is recommended to dissolve it when it will be used. It can be stored at -20°C for 12 months. The recommended final concentration is 2pmol/μl.
Transportation
Both Cas12a and 10×Buffer can be transported at room temperature.
Storage
The minimum shelf life is 1 year at room temperature for Cas12a and 1 year at -20°C for 10×Buffer.
Instruction: Protocol
SBS Genetech is recognized as one of the global major leading industry players in Gene Editing by third-party market researchers. For more details, please visit Global Gene Editing Service Market 2019 by Company, Regions, Type and Application, Forecast to 2024.
Related: Synthetic sgRNAs, Cas13a Nuclease (Lyophilized)
Only for research and not intended for treatment of humans or animals
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