All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: BST-1600 (for 1,600U)

Cat. No.: BST-16k (for 16KU)

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system. Please see Bst DNA/RNA Polymerase (glycerol-free) for more details.

Description

At SBS Genetech, we are at the forefront of offering solutions for isothermal amplification based on our world-class platform. Our Bst DNA/RNA Polymerase is at the core of this platform, which is a mixture of Bst polymerase and extremely thermostable reverse transcriptase.

Bst DNA/RNA Polymerase is suitable for isothermal amplification reaction of both DNA and RNA templates, which can detect low-sensitivity nucleic acid templates with great efficiency and specificity. Besides, with a special preparation process, this enzyme has a fast amplification rate and high tolerance to impurity. Since our Bst DNA/RNA Polymerase is extremely thermostable and also provides sensitive reverse transcriptase activity, it is reported to have higher sensitivity at high Ct values.^[1]

[1] Lu S, Duplat D, Benitez-Bolivar P, León C, Villota SD, Veloz-Villavicencio E, et al. (2022) Multicenter international assessment of a SARS-CoV-2 RT-LAMP test for point of care clinical application. PLoS ONE 17(5): e0268340. https://doi.org/10.1371/journal.pone.0268340

Contents

1,600U

• DNA/RNA isothermal amplification
• GC-rich rapid sequencing
• Rapid sequencing of micro-template DNA

Strong Recognition Ability to dUTP

Bst DNA/RNA polymerase has a strong recognition ability to dUTP. The dTTP needed for amplification reaction can be completely replaced by dUTP, so the amplification products all contain dUTP. By adding our Heat-Labile Uracil DNA Glycosylase (HL-UDG), aerosol pollutants will be completely removed in the initial reaction stage. The HL-UDG can be later inactivated irreversibly within 3 min at 65°C, which not only eliminates the pollutants but also ensures the normal amplification of nucleic acid. Therefore, the false positive caused by aerosol pollution in the reaction can be greatly reduced.

Comparison

Storage Conditions

Store the components at -20°C. Avoid multiple freeze-thaw cycles.

Additional Notes

• We provide robust and reliable solutions for the study of various diseases (African Swine Fever, COVID-19, etc) based on Bst DNA/RNA Polymerase, please contact us or email tech@sbsbio.com for more details.
• For isothermal amplification of DNA only, please see Bst DNA Polymerase.
• For the lyophilized version, please see PrimeIAmp™ Basic Lyophilized Isothermal Amplification Microbeads.

Bst DNA/RNA Polymerase is included in New Products, Science Journal, December 2, 2021. Please visit: 

https://www.science.org/doi/10.1126/science.acx9701

Instruction: Protocol

Viewpoint

Please find below the link of our viewpoint on the isothermal amplification industry, which is invited by APAC CIO Outlook, a digital and print magazine that identifies and profiles emerging companies providing cutting-edge solutions to enterprises in APAC.

SBS Genetech: Leading the Next Generation Isothermal Amplification Technology

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory