T4 UvsY Protein
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Cat. No.: UVSY-250 (for 250 μg)
Cat. No.: UVSY-1250 (for 1250 μg)
Description
The molecular weight of T4 UvsY protein is around 16KDa. This protein can stimulate the DNA-dependent ATPase activity of T4 UvsX recombinase, and lower the critical concentration of T4 UvsX recombinase that is required for activity, further promoting strand replacement. T4 UvsY protein efficiently promotes the T4 UvsX recombinase-catalyzed strand invasion reaction by recruiting to ssDNA–gp32 complexes, resulting in the release of gp32 and the binding of T4 UvsX recombinase. T4 UvsY protein is free from detectable nuclease activities.
Component
T4 UvsY protein (2.5 μg/μl, glycerol free)
Application
- DNA isothermal amplification
- RPA amplification
- Genetic testing
Heat Inactivation
60°C, 10min.
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 10% Trehalose, pH 7.5. The product does not contain glycerol and can be used to establish a freeze-drying system.
Storage
The minimum shelf life is 1 year at -20°C and 1 month at 4°C. Long-term storage at -80°C. Avoid repeated freezing and thawing.
Related: T4 UvsX Recombinase, Bsu DNA Polymerase (Large Fragment)
Only for research and not intended for treatment of humans or animals
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