T4 UvsY Protein, a phage T4 recombination mediator protein, plays an important role in T4 homologous recombination. During the recombination, T4 UvsX recombinase must compete with the prebound gp32 single-stranded binding protein for DNA-binding sites. T4 UvsY protein stimulates this filament nucleation event, promoting the combination of T4 UvsX recombinase with single-stranded DNA.
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Cat. No.: UVSY-250 (for 250 μg)
Cat. No.: UVSY-1250 (for 1250 μg)
The molecular weight of T4 UvsY protein is around 16KDa. This protein can stimulate the DNA-dependent ATPase activity of T4 UvsX recombinase, and lower the critical concentration of T4 UvsX recombinase that is required for activity, further promoting strand replacement. T4 UvsY protein efficiently promotes the T4 UvsX recombinase-catalyzed strand invasion reaction by recruiting to ssDNA–gp32 complexes, resulting in the release of gp32 and the binding of T4 UvsX recombinase. T4 UvsY protein is free from detectable nuclease activities.
T4 UvsY protein (2.5 μg/μl, glycerol free)
DNA isothermal amplification
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 10% Trehalose, pH 7.5. The product does not contain glycerol and can be used to establish a freeze-drying system.
The minimum shelf life is 1 year at -20°C and 1 month at 4°C. Long-term storage at -80°C. Avoid repeated freezing and thawing.