phi29 DNA Polymerase
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: P29P-1k (for 1,000U)
Cat. No.: P29P-20k (for 20 KU)
For an updated version that can continuously synthesize DNA at 42°C, please see phi29 HT DNA Polymerase.
The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.
We also offer phi29 DNA Polymerase in lyophilized form, please contact us for more details.
Content
1,000U
Features
- Strong strand displacement capability
- Continuous synthesis capability
- High fidelity of amplification
Applications
- DNA isothermal rolling loop amplification
- DNA synthesis requiring strong strand displacement
- Rapid replication of plasmids
- Rapid replication requiring high fidelity
Storage
Stored at -20°C for 3 years.
Notes
- 1 x phi29 Buffer: 50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 10 mM (NH4) 2SO4, 4 mM DTT. If necessary, the final concentration of 1 mM DTT and 0.2 mg/ml BSA can be added separately to improve the reaction efficiency.
- The optimum reaction temperature of the enzyme is 30℃.
- The enzyme can be inactivated at 65℃ in 10 min.
- Adjust the concentration of dNTP from 100 to 500 μM according to the type of experiments.
- The addition of Yeast Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis.
- Thio-modification at the 3' end of the primers can avoid primer degradation.
Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.
Related: phi29 HT DNA Polymerase
Only for research and not intended for treatment of humans or animals
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