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Cat. No.: P29P-1k (for 1,000U)

Cat. No.: P29P-20k (for 20 KU)

For an updated version that can continuously synthesize DNA at 42°C, please see phi29 HT DNA Polymerase.

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.

We also offer phi29 DNA Polymerase in lyophilized form, please contact us for more details.

Description

phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses an inherent 3´→ 5' proofreading exonuclease activity, thus the fidelity of the synthesized DNA fragments is very high. 3'-modified primers are highly recommended to reduce the cleavage effect of the exonuclease activity on the primer.

Features

• Strong strand displacement capability
• Continuous synthesis capability
• High fidelity of amplification

Applications

• DNA isothermal rolling loop amplification
• DNA synthesis requiring strong strand displacement
• Rapid replication of plasmids
• Rapid replication requiring high fidelity

Storage

Stored at -20°C for 3 years.

Notes

• 1 x phi29 Buffer: 50 mM Tris-HCl pH 7.5, 10 mM MgCl₂, 10 mM (NH₄) ₂SO₄, 4 mM DTT. If necessary, the final concentration of 1 mM DTT and 0.2 mg/ml BSA can be added separately to improve the reaction efficiency.
• The optimum reaction temperature of the enzyme is 30℃.
• The enzyme can be inactivated at 65℃ in 10 min.
• Adjust the concentration of dNTP from 100 to 500 μM according to the type of experiments.
• The addition of Yeast Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis.
• Thio-modification at the 3' end of the primers can avoid primer degradation.

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

Related: phi29 HT DNA Polymerase

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