FnCas12a (Cpf1)
Cat. No.: FnCas12a-100 (for 100pmol)
Cat. No.: FnCas12a-1k (for 1000pmol)
Description
FnCas12a (Cpf1) has a RuvC endonuclease domain similar to Cas9 but does not have the HNH endonuclease domain. When editing, FnCas12a does not need tracrRNA. Only crRNA is required. This is very helpful for genome editing because FnCas12a is not only smaller than Cas9 but also has a smaller crRNA molecule (nearly half of Cas9's total sgRNA). In contrast to Cas9 which needs a G-rich PAM sequence, the FnCas12a crRNA complex cleaves target DNA by recognizing T-rich PAM motifs (TTTN). The double-strand breaks of DNA after FnCas12a cleavage introduce four or five nucleotide protrudent sticky ends.
When FnCas12a recognizes and cleaves the target double-stranded DNA under the guidance of guide RNA, its "accessory cleavage" activity is activated, which can efficiently cleave non-specific single-stranded DNA (ssDNA) in the reaction system. By designing ssDNA probes with fluorescent groups or other markers at both ends, CRISPR/Cas12b can detect DNA templates and amplify the signal, to realize the detection of the target. The system has high sensitivity and strong specificity.
Applications
- Real-time Fluorescent Detection
- Colloidal gold chromatography
- Gene editing
- Molecular diagnosis
Storage
The minimum shelf life is 1 year at -20°C.
Only for research and not intended for treatment of humans or animals
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory
