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Cat. No.: ENN4-1k (for 1KU)

Cat. No.: ENN4-5k (for 5KU)

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.

Description

Endonuclease IV, also known as Nfo, is derived from E.Coli, involved in DNA damage repair. The enzyme can recognize the AP site (apurinic/apyrimidinic site) on double-stranded DNA, and cleave the first phosphodiester bond at the 5´ end of the AP site to generate 3´ hydroxyl groups and 5´ deoxyribose phosphate terminals.

In addition, Endonuclease IV also has 3´ diesterase activity, which can release glyceraldehyde phosphate, complete deoxyribose 5´ - phosphate, and phosphate from the 3´ end of DNA. The enzyme also has 3´- 5´ exonuclease activity. The gradual action of the enzyme on the substrate is affected by metal ions, DTT, EDTA, etc., and is more likely to act on double-stranded DNA at the 3´ concave end.

Component

• Endonuclease IV (10 U/μl): 100 μl
• 10X Endonuclease IV Buffer: 1 ml

Applications

• Single-cell gel electrophoresis

• SNP analysis

• Research on DNA damage and repair

Heat Inactivation

85°C, 20 min.

Unit Definition

One unit is defined as the amount of enzyme required to cleave 1 pmol of 34 mer double-stranded oligonucleotide containing one AP site at 37°C for 1h in 10 μl 1×Endonuclease IV Buffer (100 mM NaCl，50 mM Tris-HCl（pH 7.9)，10 mM MgCl2，1 mM DTT).

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.

Reagents for Recombinase Polymerase Amplification (RPA)

• T4 UvsX Recombinase
• T4 UvsY Protein
• T4 Gene 32 Protein
• Bsu DNA Polymerase
• Sau DNA Polymerase
• Exonuclease III
• Bst DNA/RNA Polymerase
• Endonuclease IV

Instruction: RPA Reaction System (for reference only)

RPA Lyophilized Kits

In addition to the previously mentioned RPA raw material enzymes, we present a varied range of kits meticulously crafted to cater to your specific requirements. All these kits are lyophilized, ensuring stability during transportation at room temperature. Delve into our exceptional selection:

Gen-1

• RPA Basic Kit (Gen-1), 
• RPA RT Basic Kit (Gen-1), 
• RPA Fluorescent Kit (Gen-1), 
• RPA RT Fluorescent Kit (Gen-1)

Gen-2 (featuring the additional introduction of a sucrose-sucrose phosphorylase energy system for enhanced stability and reliability in the amplification process)

• RPA Basic Kit (Gen-2), 
• RPA RT Basic Kit (Gen-2), 
• RPA Fluorescent Kit (Gen-2), 
• RPA RT Fluorescent Kit (Gen-2)

Gen-3 (featuring the additional introduction of the dUTP-UDG-UGI system to effectively prevent aerosol contamination)

• RPA Basic Kit (Gen-3), 
• RPA RT Basic Kit (Gen-3), 
• RPA Fluorescent Kit (Gen-3), 
• RPA RT Fluorescent Kit (Gen-3)

Our kits are meticulously crafted to deliver accurate and efficient results for your research endeavors. For further information or if you're interested in any of our products, please don't hesitate to contact us. Elevate your work with our cutting-edge solutions today!

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory