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Cat. No.: DLFS-50 (for 50T)

Description

This product employs a chromatographic double-antibody sandwich method for rapid nucleic acid amplification product detection. Compared to traditional agarose gel electrophoresis, our nucleic acid detection test strip offers simplicity in operation, swift interpretation, lacks toxic substances, and requires no equipment. When designing the initial detection sequence primer, users only need to label one primer/probe with Biotin and the other with FITC or 6-carboxyfluorescein (6-FAM). For the subsequent detection sequence primer design, one primer/probe should be labeled with Rhodamine TAMRA, while the other with Digoxin. There are two labeling options available, as detailed in the accompanying table. It is crucial to ensure that both markers can seamlessly integrate into the double-stranded amplification product simultaneously for effective detection. If employing an internal reference design, it is recommended to designate the internal reference fragment marker as digoxin.

Marking options： 

5’ Biotin-----3’ FAM(FITC), 5’ Digoxin-----3’ TAMRA

5’ Biotin----- Digoxin, 5’ TAMRA-----3’ FAM(FITC)

Instruction

1. Retrieve the appropriate number of test strips corresponding to the number of test samples, and label them on the absorbent pad. Each test strip is designed for single-use testing per sample. If the volume of the amplification product falls within the range of 50-100µL, nucleic acid detection can be directly performed in the 200µL PCR reaction tube. However, if the amplification product is less than 50µL, ultrapure water must be added to the PCR tube to adjust the volume to 50µL. Detection can only proceed after thorough mixing by pipetting.

2. Following PCR, RPA, or RAA amplification, introduce the products into the CRISPR system. Open the PCR reaction tube and carefully insert the binding pad end (indicated by an arrow) of the test strip into the PCR reaction tube (see Figure 1). Ensure that the liquid level does not exceed the top of the binding pad. Once the reading area becomes fully saturated (approximately 1-2 minutes, with potential variations due to external temperature conditions; e.g., longer saturation time may occur in colder environments such as winter), and after the appearance of the color on the quality control line (C line), remove the test strip. Interpret the test results directly based on the color development of the test strip.

3. It is imperative to read the results within 10 minutes after the color appears on the quality control line (C line). Any interpretation beyond this time frame is considered invalid.

4. Record the test outcomes, securely seal, and appropriately dispose of the test strips in a safe manner.

Storage

For optimal storage, please keep the product away from light and moisture, maintaining it within a temperature range of 4 to 30°C. The expiry date of this product is 12 months from the production date.

Instruction: Protocol

Related: 

• Universal Lateral Flow Strips
• Cas12/13 Lateral Flow Strips
• Single-Enzyme CRISPR-Based Nucleic Acid Detection Lateral Flow Test Strip
• Dual-Enzyme CRISPR-Based Nucleic Acid Detection Lateral Flow Test Strip
• Dual-Labeled Nucleic Acid Detection Lateral Flow Test Strip (Rainbow Type)

Only for research and not intended for treatment of humans or animals

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