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dNTPs Mix(10mM each)(1ml)

dNTPs Mix(10mM each)(1ml)

dNTPs Mix是由等摩尔含量的高纯度(99.5%)dATP,dCTP,dGTP和 dTTP (三蒸水钠盐溶解)组成的预混物,各自的浓度是10mM,pH值为7.5。
均不含RNase和DNase,适用于所有需要高纯度脱氧核糖核酸的分子生物学实验,例如PCR,DNA测序,cDNA合成和切口平移实验。
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目录号:EN-1

 

稳定性

             所有的dNTPs都非常稳定,我们保证自购买之日起一年内100%稳定。

 

特点

             超纯度: >99% by HPLC

             可靠:混合保证和单包装的重复性都非常好。

 

应用

             PCR 和定量PCR

             cDNA合成

             引物合成

             DNA测序

             DNA标记

             突变基因

 

质控标准

            纯度检测(HPLC) >99%

            无焦磷酸盐、DNA、RNA、DNase、RNase和DNA切口

            通过操作PCR, 定量PCR和RT-PCR 检测

 

精选引用

想了解使用我们的dNTP已发表的研究文献吗?

Visualized RNA detection of SARS-CoV-2 in a closed tube by coupling RT-PCR with nested invasive reaction

Analyst    |    4 Jan 2023    |    DOI: https://doi.org/10.1039/d2an01679f

The 20 μL reaction mixtures of the assay contained 1× visualized closed-tube PCR buffer (10 mM Tris–HCl (pH 8.5), 7.5 mM MgCl2·6H2O, 30 mM NaCl, 0.05% NP-40, 0.05% Tween-20), 50 U HiScript II reverse transcriptase, 0.25 mM dNTPs (SBS Genetech Co. Ltd, Beijing, China), 0.5 μM forward primer, 0.5 μM reverse primer, 0.25 U GoTaq DNA polymerase (Promega, Beijing, China), 3.5% PEG8000 (BSK Technology Co. Ltd, Nanjing, China), 0.1 μM UP, 0.4 μM DP, 0.2 μM hairpin probe, 100 ng of FEN1 endonuclease (prepared in our laboratory.

CRISPR/Cas genome editing perspectives for barley breeding

Psysiologia Plantarum    |    22 Apr 2022    |    DOI: https://doi.org/10.1111/ppl.13686

Primers for sgRNA of eIF4E genes were selected with WhU6 promoter region for amplification of a 362-base pair fragment: F 5′-GACCAAGCCCGTTATTCTGAC-3′, R 5′-AAGTCTGATGCAGCAAGCGAG-3′; for the region including Cas9 with the promoter: F 5′-GCTCCTGGTCCATCCACG-3′, R 5′-CGTG-GATGGACCAGGAGC-3′; for hptII: F 5′-GCTGCGCCGATGGTTTCTACA-3′, R 5′-GCCCAAAGCATCAGCTCATCG. The recommended amplification mixture contained 5 mg of the DNA template (Applied Biosystems); 2.5 mM MgCl2; 250 μM dNTPs (Beijing SBS Genetech Co., Ltd.)

Femtomolar and locus-specific detection of N6-methyladenine in DNA by integrating double-hindered replication and nucleic acid-functionalized MB@Zr-MOF

Journal of Nanobiotechnology    |    7 Dec 2021    |    DOI: https://doi.org/10.1186/s12951-021-01156-0

Klenow Fragment DNA polymerase (3′ → 5′ exo−), 10 ×  Klenow buffer (500 mM Tris–HCl, 50 mM MgCl2 and 10 mM DTT, pH 7.9), and 10 × CutSmart™ buffer (20 mM Tris–acetate, 500 mM potassium acetate, 10 mM magnesium acetate and 100 µg/mL BSA, pH 7.9) were obtained from New England Biolabs (Beijing, China). GoldView I, 20 bp DNA marker, and dATPs, dTTPs, dCTPs and dGTPs were purchased from SBS Genetech Co., Ltd., (Beijing, China)

Multiplex Visualized Closed-Tube PCR with Hamming Distance 2 Code for 15 HPV Subtype Typing

Anal. Chem.    |    22 Mar 2021    |    DOI: https://doi.org/10.1021/acs.analchem.1c00035

Reagents included GoTaq Hot Start Polymerase (Taq DNA polymerase) (Promega), flap endonuclease 1 (FEN1) prepared in our laboratory as described previously, (19) deoxynucleotide triphosphates (dNTPs) (SBS Genetech Co., Ltd., China)

An integrated electrochemical biosensor based on target-triggered strand displacement amplification and “four-way” DNA junction towards ultrasensitive detection of PIK3CA gene mutation

Biosensors and Bioelectronics    |    15 Feb 2020    |    DOI: https://doi.org/10.1016/j.bios.2019.111954

NsbI restriction enzyme, Klenow Fragment (KF) (3′→5′exo-), Nb.BbvCI, 10 × Klenow buffer (500 mM Tris-HCl, 50 mM MgCl2 and 10 mM DTT, pH 7.9) and 10 × CutSmart™ buffer (20 mM Tris-acetate, 500 mM potassium acetate, 10 mM magnesium acetate and 100 μg/mL BSA, pH 7.9) were obtained from New England Biolabs (Beijing, China). GoldViewⅠ, DNA marker and dNTP were purchased from SBS Genetech Co., Ltd (Beijing, China)

Sequence-encoded quantitative invader assay enables highly sensitive hepatitis B virus DNA quantification in a single tube without the use of a calibration curve

Royal Society of Chemistry    |    8 Aug 2019    |    DOI: https://doi.org/10.1039/c9an00970a

A virus RNA/DNA Extraction Kit was purchased from Xi'an Tianlong Science and Technology Co., Ltd (Xi'an, China), deoxynucleotide triphosphates (dNTPs) were obtained from SBS Genetech Co., Ltd (Beijing, China)

Dual cycle amplification and dual signal enhancement assisted sensitive SERS assay of MicroRNA

Analytical Biochemistry    |    1 Jan 2019    |    DOI: https://doi.org/10.1016/j.ab.2018.10.004

Klenow fragment of E.coli DNA polymerase and nicking endonuclease (NEase) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). BEAS-2B cells was purchased from GeFan Biotechnology.Go.,Ltd (Shanghai, China). Cell lysis buffer was purchased from Sangon Biotech (Shanghai, China). The mixture of four dNTPs (10 mM for each component) was purchased from SBS Genetech Co., Ltd. (Beijing, China).

 

 

仅用于研究,不用于治疗人类或动物

 

使用赛百盛产品所发表的期刊                                                                     使用赛百盛产品的大学与机构

 

 

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    北京赛百盛基因技术有限公司是中国早期提供定制寡核苷酸合成、基因合成和定制多肽合成的公司之一。自2000年成立以来,我们一直致力于利用科技创造我们引以为傲的未来。几十年来,通过提供更安全和更具性价比的产品,我们为30多个国家的研究人员提供科研服务,助力他们在生物学领域发现新的知识。
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