All products have special prices for bulk purchase, please contact us for more details if required.

Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: DFTRTD-250 (for 250U)

Cat. No.: DFTRTD-1250 (for 250U×5)

Cat. No.: DFTRTD-5k (for 1250U×4)

Cat. No.: DFTRTD-12500 (for 2500U×5)

Description

Trt DNA Polymerase is a thermostable DNA polymerase derived from a thermophilic bacterium expressed in Escherichia coli. It has a molecular weight of 94 kDa. This enzyme has been specially modified to possess strong amplification ability and extension speed. It can amplify fragments up to 3-5 kb in length, with an extension rate of 1-2 kb/min at 72°C. The enzyme exhibits 5'→3' polymerase activity, weak 5'→3' exonuclease activity, and no 3'→5' exonuclease activity. The amplified products have 3'-dA overhangs. Additionally, in the presence of Mn2+ ions, Trt DNA Polymerase exhibits reverse transcription activity. Utilizing this feature, the enzyme can be used for one-step RT-PCR, combining reverse transcription and PCR reactions in a single tube.

Our DNA-Free Trt DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-Free Trt DNA Polymerase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like RT-PCR and primer extension.

Components

• #This product does not come with RT-PCR buffer and specialized dNTPs. If conducting RT-PCR experiments, we recommend purchasing Trt DNA Polymerase from our company that includes 5× RT-PCR buffer.
• Includes a 2 mM dNTP mixture.

Activity Definition

One unit (U) of activity is defined as the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide triphosphates into an acid-insoluble fraction from marlin sperm DNA templates/primers at 72°C for 30 minutes.

Concentration

2.5 U/μl

Features

• The enzyme has been specially modified to possess strong amplification ability and extension speed, enabling efficient amplification of DNA fragments up to 3-5 kb under conditions of 2 kb/min extension.
• Excellent thermal stability: Half-life exceeds 40 minutes at 95°C.
• Tolerant to dUTP and dITP.
• PCR products have 3'-dA overhangs, suitable for direct T/A cloning.

Applications

• Conventional PCR amplification.
• Colony PCR.
• Addition of 3'-dA overhangs to PCR products for TA cloning.
• Conventional PCR following reverse transcription.

Quality Control

Relevant tests indicate the absence of exogenous endonucleases or exonucleases, as well as ribonuclease contamination. PCR analysis shows no residual host DNA, and it is capable of effectively amplifying single-copy genes in the human genome.

The enzyme's amplification activity remains stable when stored at room temperature for one week; however, it is strongly recommended to avoid long-term storage at room temperature and instead store the enzyme at -20°C after each use.

Enzyme Storage Buffer

10 mM Tris-HCl (pH 7.5 at 25°C), 300 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/ml BSA, and 50% glycerol.

Storage

This product can be stored at -20°C.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data