All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFHST-250 (for 250U)

Cat. No.: DFHST-1250 (for 1250U)

Cat. No.: DFHST-5k (for 5KU)

Cat. No.: DFHST-12500 (for 12500U)

For ligand-based hot-start DNA polymerase, please visit HM-Taq DNA Polymerase.

Description

DNA-Free HS-Taq DNA Polymerase is a HotStart Taq DNA Polymerase, activated by heating at 95°C for 5 minutes or 98°C for 2 minutes. Taq DNA Polymerase is a thermostable DNA polymerase derived from the thermophilic bacterium Thermus aquaticus, recombinantly expressed in E. coli, with a molecular weight of 94 kDa. This enzyme has been specially modified to have superior amplification capability and extension speed, capable of amplifying fragments up to 5-6 kb with an extension speed of 2-3 kb/min (72°C). It possesses 5’→3’ polymerase activity and weak 5’→3’ exonuclease activity, but lacks 3’→5’ exonuclease activity. The amplification products have 3’-dA overhangs.

Our DNA-Free HS-Taq DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free HS-Taq DNA Polymerase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Features

• Completely free from bacterial DNA.
• Versatile buffer system suitable for all applications.
• High sensitivity, enabling efficient amplification genes.
• High productivity, ensuring high yields of products.
• Avoiding nonspecific amplification and enabling room-temperature reaction setup

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

Storage

The minimum shelf life is a year at -20°C. Avoid repeated freeze-thaw cycles.

Related: HS-Taq DNA Polymerase

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory