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Cat. No.: DFHDUT-500 (for 500U)

Cat. No.: DFHDUT-2500 (for 500U×5)

Cat. No.: DFHDUT-10KU (for 2500U×4)

Cat. No.: DFHDUT-25KU (for 2500U×10)

Description

DNA-Free HS Taq-D DNA Polymerase is the hot-start version of Taq-D DNA Polymerase (KlenTaq), requiring activation by heating at 95°C for 5 minutes or at 98°C for 2 minutes. It is a recombinant, genetically engineered Taq DNA polymerase derived from the thermophilic bacterium Thermus aquaticus, with the 5' exonuclease domain deleted. The enzyme exhibits 5'→3' polymerase activity, no 5'→3' exonuclease activity, and no 3'→5' exonuclease activity. Amplification products have 3'-dA overhangs. It can amplify fragment lengths up to 3 kb with an extension rate of 1.0 kb/min (72°C). This enzyme is not suitable for TaqMan probe-based q-PCR. It is especially suitable for single nucleotide polymorphism (SNP) typing by melting curve analysis and for amplifying samples containing PCR inhibitors, such as blood.

Our DNA-Free HS Taq-D DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes Taq-D DNA Polymerase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Activity Definition

The enzyme activity is defined as the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotides into acid-insoluble material at 72°C for 30 minutes, using Mahi Mahi sperm DNA as a template/primer, and is expressed as one unit (U).

Features

• Hot-start polymerase, enhancing amplification specificity and reducing non-specific amplification and primer-dimer formation.

• More suitable for PCR of untreated blood, tissue, saliva samples compared to ordinary Taq DNA Polymerase.

• Excellent thermal stability: Half-life exceeds 40 minutes at 95°C.

• Broad tolerance range for template DNA.

• PCR products have 3'-dA overhangs, suitable for direct T/A cloning.

• Can incorporate dUTP, dITP, and fluorescently labeled nucleotides.

Applications

• Single nucleotide polymorphism (SNP) typing by melting curve analysis.

• Direct PCR of blood, tissue samples, etc.

• DNA fluorescence labeling.

Storage

Store at -20°C for one year. Avoid repeated freeze-thaw cycles

Note: All product outward appearance, the size color take the material object as. The picture only supplies the reference.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

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