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DNA-Free Taq DNA聚合酶

DNA-Free Taq DNA聚合酶

¥280.00 - ¥6,720.00
¥8,400.00
Taq DNA聚合酶最初源自水生栖热菌(Thermus aquaticus),目前通常通过其克隆基因在大肠杆菌(E. coli)中表达进行大规模生产。然而,基于大肠杆菌生产的Taq聚合酶往往残留细菌DNA,这些污染可能来源于表达载体系统或其他生产工艺。此类残留DNA可能会限制Taq DNA聚合酶在某些样本PCR反应中的应用。有研究表明,市售每单位酶中可能含有高达1,000个基因组当量的细菌DNA。相比之下,赛百盛的DNA-Free Taq DNA聚合酶几乎完全去除了生产菌株的基因组和质粒DNA残留。因此,该酶特别适用于16S和23S rDNA基因的扩增、测序与克隆,同时也能完美胜任常规PCR、引物延伸等标准实验需求。
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: DFTDP-500 (for 500U)

Cat. No.: DFTDP-2500 (for 2500U)

Cat. No.: DFTDP-10k (for 10KU)

Cat. No.: DFTDP-25k (for 25KU)

 

Description

Taq DNA Polymerase, initially derived from Thermus aquaticus, is commonly produced from its cloned gene expressed in E. coli for large-scale manufacturing. However, Taq polymerase sourced from E. coli often contains residual bacterial DNA, likely originating from the expression vector system and other manufacturing processes. This contamination can restrict the use of Taq DNA Polymerase in PCR reactions for certain samples. Some reports suggest that commercially available Taq DNA Polymerase may contain up to 1,000 genome equivalents of bacterial DNA per unit of enzyme.

Our DNA-Free Taq DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free Taq particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

 

Features

  • Completely free from bacterial DNA.
  • Versatile buffer system suitable for all applications.
  • High sensitivity, enabling efficient amplification genes.
  • High productivity, ensuring high yields of products.

 

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

 

Related: 

DNA-Free Pfu DNA Polymerase

 

 

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

 

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北京赛百盛基因技术有限公司
北京赛百盛基因技术有限公司为生命科学领域提供全方位解决方案,包括寡核苷酸合成、基因合成、多肽合成、PCR产品、恒温扩增产品、CRISPR基因编辑产品等数十条产品线,自2000年来已经为60多个国家的研究人员提供产品与服务。
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