All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFT7R-5k (for 5KU)

Cat. No.: DFT7R-25k (for 25KU)

Cat. No.: DFT7R-100k (for 100KU)

Cat. No.: DFT7R-250k (for 250KU)

Description

DNA-Free T7 RNA Polymerase is a DNA-dependent RNA polymerase encoded by the T7 bacteriophage, used for in vitro RNA synthesis. It has high specificity for the T7 phage promoter. Under the guidance of the T7 promoter, it uses DNA as a template to synthesize either sense or antisense RNA, depending on the position of the T7 promoter relative to the DNA sequence. If the DNA is downstream of the T7 promoter, T7 RNA polymerase will transcribe sense RNA; otherwise, it will transcribe antisense RNA. RNA synthesized in vitro using T7 RNA polymerase is suitable for various research and biotechnology applications, such as sgRNA for gene editing and mRNA synthesis for in vitro translation. Both double-stranded linear blunt-ended or 5’ overhang-ended DNA can serve as substrate templates for T7 RNA polymerase, making linear plasmids and PCR products suitable templates for in vitro RNA synthesis.

Our DNA-Free T7 RNA Polymerase is a recombinant protein and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.

Unit Definition

One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into acid-insoluble material within 60 minutes at 37℃ in 1× T7 RNA polymerase reaction buffer.

Features

• Completely free from bacterial DNA.
• High transcriptional activity, NTP concentration range: 0.2-10 mM.
• The T7 promoter is short and simple, making it easy to design and synthesize.

Applications

• In vitro transcription for the preparation of various RNA molecules, including mRNA, sgRNA, RNA standards, and RNA vaccine synthesis.
• Preparation of radiolabeled and fluorescently labeled RNA probes.
• Expression regulation through antisense RNA.
• Guide RNA (sgRNA) for gene editing.
• Synthesis of mRNA for in vitro translation and microinjection.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory