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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: DFT4DPE-200 (for 200U)

Cat. No.: DFT4DPE-1k (for 1000U)

The lyophilized T4 DNA Polymerase (exo-) is also available by inquiry, which can be transported at room temperature.

Description

T4 DNA polymerase, also known as T4 gp43, is a DNA polymerase enzyme sourced from the T4 bacteriophage. It has a molecular weight of 104 kDa and plays a crucial role in the synthesis of deoxyribonucleic acid (DNA) during T4 bacteriophage DNA replication. T4 DNA polymerase is responsible for the 5'→3' synthesis of both the leading and lagging DNA strands. T4 DNA polymerase exhibits stronger polymerase activity compared to DNA polymerase I but lacks 5'-3' exonuclease activity. Our T4 DNA polymerase (exo-) has been modified through a point mutation (D219A) to significantly reduce its 3'-5' exonuclease activity while retaining its polymerase activity. Our T4 DNA polymerase is recombinantly expressed and purified through multiple steps.

Our DNA-Free T4 DNA Polymerase (exo-), on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free T4 DNA Polymerase (exo-) particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Unit Definition

One unit of activity is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into an acid-insoluble material at 37°C within 30 minutes.

Activity Assay Conditions

1× T4 DNA polymerase (exo-) Buffer: 20 mM Tris-HCl, 50 mM NaCl, 10 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA (pH 7.9 @ 25°C), incubated at 37°C.

Features

• No strand displacement activity.
• Lacks 5'-3' exonuclease activity, preventing excision translation.
• Significant reduction in 3'-5' exonuclease activity.
• Heat inactivation at 75°C for 20 minutes.

Applications

• Gap filling (no gap translation activity).
• Removal of 3' overhang single-stranded DNA or filling 5' overhang single-stranded DNA to create blunt ends.
• Generation of 5' overhangs in seamless cloning for complementary pairing between genes and vectors.

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freeze-thaw cycles.

Related: T4 DNA Polymerase

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data