All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFSAULF-250 (for 250U)

Cat. No.: DFSAULF-1250 (for 1250U)

The glycerol-free version is also available for this product, which can be used to establish a freeze-drying system.

Description

DNA-Free Sau DNA Polymerase (Large Fragment) is derived from the DNA polymerase (Sau) I gene of Staphylococcus aureus, which is obtained by cutting 1-293 AA at its N-terminal. This enzyme retains the 5’-3‘ polymerase activity of DNA polymerase but lacks 5‘ - 3’ and 3‘ - 5’ exonuclease activities, which can be used for recombinase amplification.

DNA-Free Sau DNA Polymerase (Large Fragment) is a DNA polymerase with strand displacement activity, which can be used for recombinase isothermal amplification. The protein-DNA complex formed by the combination of recombinant enzyme UvsX and primers can find homologous sequences in double-stranded DNA. Once the primer locates the homologous sequence, a strand displacement reaction will occur. Sau DNA polymerase starts DNA synthesis, and exponentially amplifies the target region on the template. The replaced DNA strand will bind to SSB to prevent further replacement.

Our DNA-Free Sau DNA Polymerase (Large Fragment), on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-Free Sau DNA Polymerase (Large Fragment) particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes.

Applications

• Primer labeling
• Second strand cDNA synthesis
• DNA strand displacement synthesis
• Single dA tailing

Heat Inactivation

75°C, 20 min.

Unit Definition

One unit is defined as adding 10 nmol acidic refractory substance in 30 min at 37°C.

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freezing and thawing.