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DNA-Free Pfu DNA聚合酶

DNA-Free Pfu DNA聚合酶

¥1,120.00 - ¥36,750.00
Pfu DNA聚合酶通过从携带激烈火球菌(Pyrococcus furiosus)DNA聚合酶克隆基因的大肠杆菌中分离纯化获得。该酶在镁离子存在条件下,可催化核苷酸沿5´→3´方向聚合形成双链DNA,并具有3´→5´外切核酸酶(校对)活性,特别适用于需要高保真度的PCR和引物延伸反应。赛百盛推出的DNA-Free Pfu DNA聚合酶采用先进纯化工艺,几乎完全去除了生产菌株的基因组及质粒DNA残留。该酶特别适用于16S和23S rDNA基因的扩增、测序与克隆,同时也能完美胜任常规PCR、引物延伸等标准实验需求。
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: DFPDP-250 (for 250U)

Cat. No.: DFPDP-1250 (for 1250U)

Cat. No.: DFPDP-5k (for 5KU)

Cat. No.: DFPDP-12500 (for 12500U)

 

Description

Pfu DNA polymerase is isolated and purified from E. coli, which carries the cloned gene of Pyrococcus furiosus DNA polymerase. This enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and exhibits 3´→5´ exonuclease (proofreading) activity. Pfu DNA polymerase is recommended for PCR and primer extension reactions that require high fidelity.

Our DNA-Free Pfu DNA Polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free Pfu particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

 

Features

  • Completely free from bacterial DNA.
  • Versatile buffer system suitable for all applications.
  • High sensitivity, enabling efficient amplification genes.
  • High productivity, ensuring high yields of products.

 

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.

 

Related: DNA-Free Taq DNA Polymerase

 

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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北京赛百盛基因技术有限公司
北京赛百盛基因技术有限公司为生命科学领域提供全方位解决方案,包括寡核苷酸合成、基因合成、多肽合成、PCR产品、恒温扩增产品、CRISPR基因编辑产品等数十条产品线,自2000年来已经为60多个国家的研究人员提供产品与服务。
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