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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: DFIECP-10 (for 10U)

Cat. No.: DFIECP-50 (for 50U)

Description

Inorganic Pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to generate orthophosphate: P2O7^4- + H2O → 2HPO4^2-. It is primarily used to degrade the byproduct pyrophosphate in nucleic acid synthesis reactions. This action alleviates the inhibitory effect of the byproduct on nucleic acid synthesis reactions, leading to an increase in synthesis yield. Our Inorganic E.coli Pyrophosphatase is recombinantly expressed and purified through multiple steps.

Our DNA-Free Inorganic E.coli Pyrophosphatase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-Free Inorganic E.coli Pyrophosphatase particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes.

Unit Definition

One unit of activity is defined as the amount of enzyme required to catalyze the production of 1 μmol of phosphate per minute at 37°C in a 1× inorganic pyrophosphatase reaction buffer.

Activity Assay Conditions

1× Buffer: 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, and 2 mM PPi.

Applications

• Enhances RNA yield in in vitro transcription reactions.
• Increases DNA synthesis yield in PCR and isothermal nucleic acid amplification reactions.

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freeze-thaw cycles.

Related:

• Thermostable Inorganic Pyrophosphatase
• Inorganic E.coli Pyrophosphatase
• Inorganic Yeast Pyrophosphatase