All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: DFHLDP-250 (for 250U)

Cat. No.: DFHLDP-1250 (for 1250U)

Cat. No.: DFHLDP-5k (for 5KU)

Cat. No.: DFHLDP-12500 (for 12500U)

Description

DNA-Free HS LATaq DNA polymerase is a HotStart LATaq DNA polymerase, which requires activation by heating at 95℃ for 5 minutes or 98℃ for 2 minutes. LATaq DNA polymerase is a genetically engineered enzyme based on Taq polymerase, containing a double-stranded DNA-binding protein segment that significantly enhances the polymerase's extension capability and speed. Therefore, LATaq DNA polymerase is highly suitable for amplifying long DNA fragments, with fragment lengths reaching 5-8 kb and an extension speed of 3-4 kb/min (at 72℃). This enzyme is a recombinant DNA polymerase expressed in Escherichia coli, with a molecular weight of 107 kDa. It exhibits 5’→3’ polymerase activity, weak 5’→3’ exonuclease activity, and no 3’→5’ exonuclease activity. The amplification products have 3'-dA overhangs.

Our DNA-Free HS LATaq DNA polymerase, on the other hand, is devoid of genomic and plasmid DNA from the production strain. This makes DNA-free Pfu particularly suitable for amplifying, sequencing, and cloning 16S and 23S rDNA genes, as well as for standard applications like PCR and primer extension.

Unit Definition

The amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acid-insoluble material within 30 minutes at 72℃ using salmon sperm DNA as the template/primer is defined as one unit of activity (U).

Features

• Completely free from bacterial DNA.
• This enzyme is a hot-start polymerase, which improves amplification specificity and reduces non-specific amplification and primer dimer formation.
• Specially modified for strong amplification capability, effectively amplifying 3-5 kb DNA fragments under 4 kb/min extension conditions.
• Improved thermal stability, allowing pre-denaturation at 98℃ for 2 minutes.
• Suitable for two-step PCR at 60-72℃, reducing overall PCR time.
• PCR products have 3’-dA overhangs, suitable for T/A cloning.
• Capable of incorporating dUTP, dITP, and fluorescently labeled nucleotides.

Applications

• Hot-start PCR amplification.
• Multiplex PCR.
• Colony PCR.
• T/A cloning of PCR products with 3’-dA overhangs.
• DNA fluorescence labeling.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory