CRISPR Cas12a-RPA Lyophilized RNA Detection Kit (Two-Step)
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Cat. No.: C12ALRT-96 (for 96T)
Cat. No.: C12ALRT-480 (for 480T)
Description
Cas12a forms a functional complex with crRNA and "slides" along the target nucleic acid sequence. Upon successful pairing, Cas12a is specifically activated and trans-cleaves surrounding reporter molecules. This kit combines isothermal amplification technology with Cas12a, featuring high sensitivity, high specificity, and a high signal-to-noise ratio. It is widely used in molecular diagnostics, enabling rapid and accurate detection of pathogens.
Kit Components
No. 1 | Item Reaction Buffer (2X) | Size 1000 μL |
2 | Core Mix (4X) | 500 μL |
3 | Positive Control (10X) (includes primer and DNA template) | 30 μL |
4 | Cleavage Buffer (10X) | 240 μL |
5 | Cas12a Protein (10 μM) | 20 μL |
6 | crRNA for Positive Control (20X) | 20 μL |
7 | Reporter (4 μM) | 80 μL |
8 | Starter (10X) | 200 μL |
Additional Required Materials
- Fluorometer for reading FAM signals (e.g., qPCR instrument)
- Pipettes
- Nuclease-free water
- Target sequence-specific primers for isothermal amplification
- crRNA/gRNA: Forms a functional complex with LbaCas12a and is specifically activated by the target sequence. (LbaCas12a crRNA scaffold sequence: 5’-UAAUUUCUACUAAGUGUAGAU-3’)
Application
The CRISPR Cas12a DNA Detection Kit (Two-Step) is used for rapid DNA detection. First, perform isothermal amplification, then the amplification products activate Cas12a, generating a fluorescent signal. This enables rapid detection of DNA pathogens.
Related:
- CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas12a-RPA Lyophilized DNA Detection Kit (Two-Step)
- CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (Two-Step)
- CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas12a-RPA RNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas12a-RPA Lyophilized RNA Detection Kit (Two-Step)
- CRISPR Cas12a-RPA RNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (Two-Step)
- CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- Lateral Flow Assay for CRISPR SHERLOCK
Only for research and not intended for treatment of humans or animals
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