Cas9 Nuclease is a recombinant Streptococcus pyogenes Cas9 (wt) protein. A nucleic localization signal (NLS) is added which can be used for genome editing by inducing site-specific DNA double-stranded breaks. Cas9 protein can form ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the NLS.
Cat. No.: NCas-500
Compared with the mRNA or plasmid systems:
Transcription and translation processes are not required
Avoiding the risk of inserting foreign DNA into the genome
Minimizing the off target effect
in vitro cleavage assay
RNP complex transfection
Recommended to be stored at -20°C.
Only for research and not intended for treatment of humans or animals