AsCas12a (Cpf1), a novel tool for gene detection and imaging, has a similar function as Cas9 with a smaller size.
Cat. No.: AsCas12a-100 (for 100pmol)
Cat. No.: AsCas12a-1k (for 1000pmol)
NLS (nuclear localization sequence) modification is also available if required.
AsCas12a (Cpf1) has a RuvC endonuclease domain similar to Cas9 but does not have the HNH endonuclease domain. When editing, AsCas12a does not need tracrRNA. Only crRNA is required. This is very helpful for genome editing because AsCas12a is not only smaller than Cas9 but also has a smaller crRNA molecule (nearly half of Cas9's total sgRNA). In contrast to Cas9 which needs a G-rich PAM sequence, the AsCas12a crRNA complex cleaves target DNA by recognizing T-rich PAM motifs (TTTN). The double-strand breaks of DNA after AsCas12a cleavage introduce four or five nucleotide protrudent sticky ends.
When AsCas12a recognizes and cleaves the target double-stranded DNA under the guidance of guide RNA, its "accessory cleavage" activity is activated, which can efficiently cleave non-specific single-stranded DNA (ssDNA) in the reaction system. By designing ssDNA probes with fluorescent groups or other markers at both ends, CRISPR/Cas12b can detect DNA templates and amplify the signal, to realize the detection of the target. The system has high sensitivity and strong specificity.
Real-time Fluorescent Detection
Colloidal gold chromatography
The minimum shelf life is 1 year at -20°C.
Only for research and not intended for treatment of humans or animals