2×Taq PCR MasterMix（5 × 1mL）
目录号：TPM-5
包装规格
本产品为1mL×5支包装，可进行50ul PCR反应体系200次反应，或20ul反应体系500次。
使用说明:
1.冰浴中彻底融化2×MasterMix，混匀后短暂离心。
2.我们强烈建议客户购买本产品后分装成小份避免反复冻融。
3. 以50μl反应体系为例，按照下表在0.2ml PCR管中加入下列成分
4. 短暂离心将反应液收集到管底。
5. 若基因扩增仪不防蒸发，请加1-2滴石蜡油。
6. 在PCR仪上执行以下程序（仅供参考）
注：在实际操作中需根据模板的结构、扩增片段的大小和引物的长短等具体情况设定反应条件（温度、时间等）。
精选引用
对于使用我们的Taq DNA聚合酶的已发表研究感兴趣吗？
Pseudomonas spp. associated with tomato pith necrosis in the Salto area, Northwest Uruguay
European Journal of Plant Pathology | 19 Jan 2023 | DOI: https://doi.org/10.1007/s10658-023-02639-6
The PCR reactions contained 1X PCR buffer, 2.5 mM MgCl2, 0.4 mM of each dNTP, 0.4 μM of each primer, 1 U Taq polymerase (SBS Genetech Co., Ltd., China), and 1 μL of template DNA (100 ng μl−1). The PCR reaction was adjusted to a final volume of 25 μl with MQ water.
Sequencing of Norovirus in Southern, Nigeria: Prevalent Genotypes and Putative GII.4 Novel Recombinants among Children
Genetic Variation | 16 Dec 2020 | DOI: https://doi.org/10.5772/intechopen.94389
The RT-PCR used is a very sensitive method, it can detect as few as 5 x 106 copies per gram of stool sample. U-TaQ DNA polymerase (SBS genetech, Beijing, China), a high-fidelity thermostable enzyme that can withstand prolonged incubation at high temperature up to 95°C without significant loss of activity was used for this RT-PCR protocol.
Semi-nested polymerase chain reaction over blood culture in detection of bloodstream fungal infection in leukemic children with febrile neutropenia
Journal of Applied Hematology | 17 Nov 2020 | DOI: https://doi.org/10.4103/joah.joah_41_20
Taq polymerase enzymes and customized primers were procured from SBS Genetech Co., Ltd., China.
Expression, purification and initial characterization of human serum albumin domain I and its cysteine 34
PLOS ONE | 12 Oct 2020 | DOI: https://doi.org/10.1007/s10658-023-02639-6
Total RNA from HepG2 cells was isolated using the RNeasy mini kit (QIAGEN) following manufacturer’s instructions. Retrotranscription was performed using 2.5 μg of RNA, a specific HSA oligonucleotide (ATAAGCCTAAGGCAGCTTGACTGG) and Superscript II reverse transcriptase (Invitrogen). The full-coding sequence of HSA was PCR- amplified with U-Taq (SBS GeneTech)
Evaluation of Nested broad-range PCR for Pathogen Detection in Negative Blood Cultures
JOURNAL OF CLINICAL RESEARCH AND APPLIED MEDICINE | 6 Oct 2020 | DOI: https://doi.org/10.5530/jcram.2.2.11
Taq polymerase enzymes and customized primers were procured from SBS Genetech Co., Ltd., China.
Identificación por catálogo y detección molecular de bovinos Holstein portadores de braquiespina en Uruguay
Revista FAVE. Sección Ciencias veterinarias | 1 Aug 2020 | DOI: https://doi.org/10.14409/favecv.v19i2.9523
La reacción fue puesta a punto en un volumen final de 25µL conteniendo: 100ng de ADN genómico, 2.5µL de Buffer de PCR 10X (Mg2+: 20mM), 1µM de cada primer, 10mM dNTPs y 0.4µL U-Taq ADN polimerasa (SBS Genetech Co., Ltd., China).
仅用于研究，不用于治疗人类或动物
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