目录号：TPEM-5

产品概述

Taq PCR EasyMasterMix是我们为科研人员常规PCR实验提出的基础解决方案。

本产品包含Taq DNA聚合酶、dNTPs、MgCl2、反应缓冲液，浓度为1×。其中Taq DNA聚合酶是从带有Thermus aquaticus (Taq) DNA聚合酶基因的大肠杆菌中经诱导表达后分离提纯得到的，其分子量为94 KD。Taq DNA聚合酶具有5′→3′DNA聚合酶活性和5′→3′外切核酸酶活性，无3′→5′外切酶活性。在PCR反应中，Taq DNA聚合酶延伸速度为1-2 kb/min。

由于EasyMasterMix系列浓度均为1×，只需加入引物和模板即可进行扩增，无需补水。进一步减少了移液操作，最大限度提高了通量和结果的重现性。EasyMasterMix系列本身含有蓝紫色染料，扩增完成后可直接上样进行电泳检测。扩增产物3'端带A，可以直接用于T/A载体克隆。

使用说明

1.冰浴中彻底融化1×EasyMasterMix，混匀后短暂离心。

2.我们强烈建议客户购买本产品后分装成小份避免反复冻融。

3.以50μl反应体系为例，按照下表在0.2ml PCR管中加入下列成分

4. 短暂离心将反应液收集到管底。

5. 若基因扩增仪不防蒸发，请加1-2滴石蜡油。

6. 在PCR仪上执行以下程序（仅供参考）

注：在实际操作中需根据模板的结构、扩增片段的大小和引物的长短等具体情况，设定最佳的反应条件（温度、时间等）。

精选引用

对于使用我们的Taq DNA聚合酶的已发表研究感兴趣吗？

Pseudomonas spp. associated with tomato pith necrosis in the Salto area, Northwest Uruguay

^{_{European Journal of Plant Pathology    |     19 Jan 2023    |    DOI: https://doi.org/10.1007/s10658-023-02639-6}}

^{_{The PCR reactions contained 1X PCR buffer, 2.5 mM MgCl2, 0.4 mM of each dNTP, 0.4 μM of each primer, 1 U Taq polymerase (SBS Genetech Co., Ltd., China), and 1 μL of template DNA (100 ng μl−1). The PCR reaction was adjusted to a final volume of 25 μl with MQ water.}}

Sequencing of Norovirus in Southern, Nigeria: Prevalent Genotypes and Putative GII.4 Novel Recombinants among Children

^{_{Genetic Variation    |    16 Dec 2020    |    DOI: https://doi.org/10.5772/intechopen.94389}}

^{_{The RT-PCR used is a very sensitive method, it can detect as few as 5 x 106 copies per gram of stool sample. U-TaQ DNA polymerase (SBS genetech, Beijing, China), a high-fidelity thermostable enzyme that can withstand prolonged incubation at high temperature up to 95°C without significant loss of activity was used for this RT-PCR protocol.}}

Semi-nested polymerase chain reaction over blood culture in detection of bloodstream fungal infection in leukemic children with febrile neutropenia

^{_{Journal of Applied Hematology    |    17 Nov 2020    |    DOI: https://doi.org/10.4103/joah.joah_41_20}}

^{_{Taq polymerase enzymes and customized primers were procured from SBS Genetech Co., Ltd., China.}}

Expression, purification and initial characterization of human serum albumin domain I and its cysteine 34

^{_{PLOS ONE    |    12 Oct 2020    |    DOI: https://doi.org/10.1007/s10658-023-02639-6}}

^{_{Total RNA from HepG2 cells was isolated using the RNeasy mini kit (QIAGEN) following manufacturer’s instructions. Retrotranscription was performed using 2.5 μg of RNA, a specific HSA oligonucleotide (ATAAGCCTAAGGCAGCTTGACTGG) and Superscript II reverse transcriptase (Invitrogen). The full-coding sequence of HSA was PCR- amplified with U-Taq (SBS GeneTech)}}

Evaluation of Nested broad-range PCR for Pathogen Detection in Negative Blood Cultures

^{_{JOURNAL OF CLINICAL RESEARCH AND APPLIED MEDICINE   |    6 Oct 2020    |    DOI: https://doi.org/10.5530/jcram.2.2.11}}

^{_{Taq polymerase enzymes and customized primers were procured from SBS Genetech Co., Ltd., China.}}

Identificación por catálogo y detección molecular de bovinos Holstein portadores de braquiespina en Uruguay

^{_{Revista FAVE. Sección Ciencias veterinarias   |    1 Aug 2020    |    DOI: https://doi.org/10.14409/favecv.v19i2.9523}}

^{_{La reacción fue puesta a punto en un volumen final de 25µL conteniendo: 100ng de ADN genómico, 2.5µL de Buffer de PCR 10X (Mg2+: 20mM), 1µM de cada primer, 10mM dNTPs y 0.4µL U-Taq ADN polimerasa (SBS Genetech Co., Ltd., China).}}

仅用于研究，不用于治疗人类或动物

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